Applications of Fourier transform infrared spectroscopy, Fourier transform infrared microscopy and near-infrared spectroscopy to cancer research

Kazuyuki Yano,Kuniyoshi Kumaido,Hiroo Katayama,Narumi Hirosawa,Yasushi Sakamoto,Shouko Tonooka,Akira Satomi

doi:10.1155/2003/329478

Abstract

Glycogen levels in human lung and colorectal cancerous tissues were measured by the Fourier transform (FT-IR) spectroscopic method. Reliability of this method was confirmed by chemical analyses of the same tissues used for the FT-IR spectroscopic measurements, suggesting that this spectroscopic method has a high specificity and sensitivity in discriminating human cancerous tissues from noncancerous tissues. The glycogen levels in the tissues were compared with the clinical, histological and histopathological factors of the cancer, demonstrating that glycogen is a critical factor in understanding the biological nature of neoplastic diseases. Furthermore, direct measurement of a very small amount of tissue by a FT-IR microscope suggested that it could be used as a diagnostic instrument for various tissue samples obtained via a fine needle biopsy procedure. The progressive alterations in rat mammary gland tumors were investigated by a near-infrared (NIR) spectrometer with a fiber optic probe. A lipid band due to the first overtone ofn-alkane was used to quantitatively evaluate malignant changes in the tumors. NIR spectroscopy may offer the potential for non‒invasive,in vivodiagnosis of human cancers.

Highlights

Infrared radiation is divided into three regions, 14,000 to 4,000 cm−1 (714 to 2500 nm) for nearinfrared, 4000 to 400 cm−1 (2.5 to 25 μm) for mid-infrared, and 400 to 4 cm−1 (25 to 2500 μm) for far-infrared [1]
Yano et al / Fourier transform infrared (FT-IR) spectroscopy, microscopy, and NIR spectroscopy for cancer research transform microscopy (FT-IR-MC) has received a great amount of attention from various aspects since it allows highly precious analysis of very small sections of tissue in situ without extraction [6,7,8]
We studied glycogen levels in human lung tissues in relation to the histological classification and differentiation of carcinomas by an attenuated total reflectance FT-IR spectroscopic method [4]: A tissue sample (100 mg) was pulverized under liquid nitrogen using a small pre-cooled mortar and pestle

Summary

Introduction

Infrared radiation is divided into three regions, 14,000 to 4,000 cm−1 (714 to 2500 nm) for nearinfrared, 4000 to 400 cm−1 (2.5 to 25 μm) for mid-infrared, and 400 to 4 cm−1 (25 to 2500 μm) for far-infrared [1]. Fourier transform infrared (FT-IR) spectroscopy, which is a non-invasive physical method, has received attention for investigation of biomolecules and for understanding the biological nature of neoplastic diseases [2,3,4,5]. K. Yano et al / FT-IR spectroscopy, microscopy, and NIR spectroscopy for cancer research transform microscopy (FT-IR-MC) has received a great amount of attention from various aspects since it allows highly precious analysis of very small sections of tissue in situ without extraction [6,7,8]. Diagnostic applications of the mid-infrared spectroscopy, are limited because many biological materials absorb the mid-infrared radiation so strongly that the path lengths greater than 10–15 μm are not used. We would like to review applications of these apparatus to cancer research through our works

FT-IR spectroscopic study of human lung and colorectal cancers

FT-IR microscopic study of human lung cancer

In vivo NIR spectroscopic study of rat mammary gland tumors

Conclusion