Abstract
Glycogen levels in the carcinoma and normal tissues of human lungs were studied by the FT-IR method using the integrated area ratios of glycogen to protein (A1045/A1545). Further amounts of glycogen and protein contained in the same tissues were measured by an anthrone method and by using a dotMETRIC™ kit, respectively. Ratios of glycogen to protein obtained here were compared with the FT-IR spectroscopic data in relation to abnormality and histological classification of the tumors indicating a good correlation between them. The present results show that the FT-IR method is very reliable and that may be of wide application for studying glycogen levels in various tissues as well as diagnosis purpose.
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