Abstract
Three-dimensional cell culture is widely recognized as a model in vitro that can better simulate the interaction between tumor cells in vivo and cell microenvironment. Compared with the traditional two-dimensional cell culture, it has obvious advantages in the study of the observation of tumor cells biological behavior, tumor resistance, radiation insensitivity, gene expression in vivo and in other aspects of the study. It can provide a valuable model in vitro for experimental study on the basis of tumor. Key words: Neoplasm metastasis; Cell culture techniques; Tumor microenvironment
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