Abstract
A method is described for the separation and identification of fluorescein and four brominated derivatives on polyamide layers. The best separation was carried out by development with a solvent system composed of methanol-ammonia-water (10 : 2 : 88) and Rf values obtained thereby decreased as bromine number of the components increased. By the method subsidiary colors in commercial dibromofluoresceins were identified successfully, and furthermore determined by fluorometry after extraction from the layers.
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