Abstract
A new technique is described for simultaneously measuring, within 100 μs, cell volume, membrane breakdown voltage, and internal conductivity on individual cells of microscopic dimensions. The system is a modification of the hydrodynamically focusing Coulter Counter®. Tumor cells established from rat fetal brain cells of average volume 2100 μm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> were measured. A breakdown voltage of 0.86 V was found with an apparent volume underestimation after breakdown of 40 percent.
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