Abstract
In the present study we investigated phospholipid bilayer membranes and phospholipid vesicles made from dioleoylphosphatidylcholine (DOPC) or its mixture with the phosphate ester derivative of long-chain polyprenol (hexadecaprenyl diphosphate, C 80-PP) by electrophysiological and transmission electron microscopy (TEM) techniques. The membrane conductance-temperature relationships and the membrane breakdown voltage have been measured for different mixtures of C 80-PP/DOPC. The current-voltage characteristics, the membrane conductance, the activation energy of ion migration across the membrane and the membrane breakdown voltage were determined. Hexadecaprenyl diphosphate decreases the membrane conductance, increases the activation energy and the membrane breakdown voltage for the various values of C 80-PP/DOPC mole ratio. The analysis of TEM micrographs shows several characteristic structures, which have been described. The data indicate that hexadecaprenyl diphosphate modulates the surface curvature of the membranes by the formation of aggregates in liquid-crystalline phospholipid membranes. The properties of modified membranes can result from the presence of the negative charges in the hydrophilic part of C 80-PP molecules and can be modulated by the concentration of this compound in membranes. We suggest that the dynamics and conformation of hexadecaprenyl diphosphate in membranes depend on the transmembrane electrical potential.
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