Abstract
The analysis of the main germ plasm resources of Morinda officinalis How. in Zhaoqing area and the definition of the relationship among the varieties will provide scientific basis for the cultivation, protection and identification of Morinda officinalis How.. SRAP and CDDP molecular markers were used to analyze the genetic diversity of 9 Morinda officinalis How. materials in Zhaoqing. The genetic similarity coefficients among samples were calculated by NTSYS-pc2.1 software, cluster analysis was carried out according to UPGMA method, and the results of two kinds of molecular markers were compared and analyzed. 28 pairs of SRAP primers and 16 CDDP primers were used to amplify 140 and 83 bands respectively, of which the number of polymorphic bands was 68 and 63 respectively, the polymorphism ratio (PPB) was 48.6% and 75.9% respectively, the genetic similarity coefficient varied from 0.69 to 0.96 and 0.53 to 0.95, and the average genetic similarity coefficient was 0.83 and 0.69 respectively. When the genetic similarity coefficient of SRAP was 0.86 and that of CDDP was 0.71, the four varieties of Guangning special type, Guangning special grafting type, local large leaf type and local lobular type were grouped into one group. SRAP and CDDP molecular markers can be used for genetic diversity analysis of Morinda officinalis How.. There are some differences between SRAP and CDDP, so the combined analysis of the two kinds of markers can more accurately reveal the genetic relationship between Morinda officinalis How. germplasm. The results of CDDP clustering were more consistent with those of combined analysis, indicating that CDDP was more suitable for genetic diversity analysis of Morinda officinalis How. than SRAP.
Highlights
Morinda officinalis How. is a woody vine medicinal plant of Morinda in Rubiaceae, which is used as medicine with its fleshy dry root because it’s sweet in taste and mild in nature
The results showed that the two molecular markers could amplify abundant polymorphic bands, but Conserved DNA-derived polymorphism (CDDP) marker was better than Sequence-related amplified polymorphism (SRAP) marker in revealing the genetic diversity of Morinda officinalis How
The results of the two molecular markers both showed that the genetic distance between local large leaf type and local lobular type of Deqing was the closest, and the Guangning special type and the Guangning special grafting type were closely clustered, indicating that the two molecular markers could mutually confirm the accuracy of the genetic diversity analysis of Morinda officinalis How. germplasm
Summary
Morinda officinalis How. is a woody vine medicinal plant of Morinda in Rubiaceae, which is used as medicine with its fleshy dry root because it’s sweet in taste and mild in nature. Sequence-related amplified polymorphism (SRAP) is a new type of molecular markers based on PCR (Sun et al, 2010). SRAP use double primers to amplify open reading frames (ORFs) and detect their polymorphisms, which has the advantages of universal primers, simple operation, high codominance, and no need to predict the sequence information of the material (Zhang et al, 2011; Yang et al, 2016). There are no reports on SRAP and CDDP molecular markers of Morinda officinalis How. Morinda officinalis How. has high requirements for planting environment and long growth cycle, so there is few artificial planting and it is in short supply on the market. In order to protect the germplasm resources of Morinda officinalis How., accurately identify its varieties, and cultivate excellent strains, SRAP and CDDP molecular markers were used to analyze the genetic diversity of 9 Morinda officinalis How. materials in Zhaoqing, which has important scientific significance
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