SRAP Polymorphism of Space-Flight Mutation Sugarbeet SP3 Generation Breeding Material

Abstract

The SRAP molecular marker method was used to analyze the genetic diversity of 33 materials, and the yield and quality of some materials were identified. Molecular marker analysis showed that: 42 pairs of primer combinations were used to generate a total of 715 amplification bands, of which 320 bands were polymorphic, the average polymorphic bands rate was 44.7%. The average genetic distance was 0.3942 and average genetic similarity coefficient was 0.6748. UPGMA method was used to carry out cluster analysis, tetraploid polyembryoby material, diploid polyembryony material, diploid monogerm material showed clustering trend, respectively. The genetic distance of HT5-207-1-1 of HT5-207 strain in diploid polyembryony material was the smallest compared with the control (0.2756), the genetic similarity coefficient was the largest (0.7591), i.e. the degree of variation was the smallest. The genetic distance of HT8-86-1 of HT8-86 strain of diploid monogerm material was the largest compared with the control (0.4646), the genetic similarity coefficient was the smallest (0.6284), i.e. the degree of variation was the largest. Yield and quality evaluation results showed that the root yield of space-flight mutation diploid material showed significantly increased trend compared with the original control, but sugar content was decreased slightly, while the root yield and sugar content of tetraploid materials showed slightly lower trend compared with the original control. Studies have shown that the space-flight mutation could contribute to sugar beet a certain degree of variation, and had significant effect to screen diploid innovative high-yield material.