Abstract
Triadimenol, triadimefon and tetraconazole were simultaneously determined in human urine by coupled-column liquid chromatography with both UV and ES-MS detection. These procedures were validated and the values of some merit figures, such as, linear range, detection limits, precision and recovery, obtained with both methods, are compared. A 5 μm GFF-II, internal-surface, reversed-phase (ISRP, Pinkerton) column coupled to a 3 μm reversed-phase, Microsphere C18 column were used. To increase sensitivity a solid-phase extraction step with C18 cartridge was carried out yielding recoveries 80–107% (n=5) with RSD 3–8% for the LC-LC-UV method and 88–108% (n=5), RSD 5–11% for the LC-ES-MS method. Limits of detection were 1.5–3.5 μg L−1 for LC-LC-UV and 0.5–1.0 μg L−1 for LC-ES-MS. The methods were employed to detect and quantify pesticides in urine of pest control operators and non-occupationally exposed subjects.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
