Application of reactive dyes in Validation of favipiravir from pharmaceutical dosages

Abstract

Simple sensitive and accurate ion pair complex formation with reactive dyes, extractive spectrophotometric methods have developed for the estimation of favipiravir in pharmaceutical dosage form. The methods are based on the formation of ion paired coloured complexes by the drug with reactive dyes like Congo red, eriochrome black T and methyl orange in acidic medium. The ion associated complexes were formed and quantitatively extracted under the experimental condition in chloroform. The absorbance values were measured at 490 nm, 500 nm, and 430 nm respectively. The proposed methods were validated statistically. Recoveries of methods were carried out by standard addition method. The linearity was found to be 5.0-30.0 μg/ml, 5 -12 μg/ml, and 2-20 μg/ml for methods respectively. The low values of standard deviation and percentage RSD indicate high precision of methods. Hence these methods are useful for routine estimation of favipiravir in pharmaceutical dosages.