Abstract
Zinc oxide (ZnO) nanostructures are widely used in optical sensors and biosensors. Functionalization of these nanostructures with polymers enables optical properties of ZnO to be tailored. Polydopamine (PDA) is a highly biocompatible polymer, which can be used as a versatile coating suitable for application in sensor and biosensor design. In this research, we have grown ZnO-based nanorods on the surface of ITO-modified glass-plated optically transparent electrodes (glass/ITO). Then the deposition of the PDA polymer layer on the surface of ZnO nanorods was performed from an aqueous PDA solution in such a way glass/ITO/ZnO-PDA structure was formed. The ZnO-PDA composite was characterized by SEM, TEM, and FTIR spectroscopy. Then glucose oxidase (GOx) was immobilized using crosslinking by glutaraldehyde on the surface of the ZnO-PDA composite, and glass/ITO/ZnO-PDA/GOx-based biosensing structure was designed. This structure was applied for the photo-electrochemical determination of glucose (Glc) in aqueous solutions. Photo-electrochemical determination of glucose by cyclic voltammetry and amperometry has been performed by glass/ITO/ZnO-PDA/GOx-based biosensor. Here reported modification/functionalization of ZnO nanorods with PDA enhances the photo-electrochemical performance of ZnO nanorods, which is well suited for the design of photo-electrochemical sensors and biosensors.
Highlights
Materials and InstrumentsExperimental sodium sulfate, zinc nitrate hexahydrate, and phosphate-buffered saline (tablet) were pur2.1
Introduction published maps and institutional affilPhotoelectrochemical (PEC) detection is a fast-developing technology [1,2]
We have reported UV light photoinduced processes occurring at the Zinc oxide (ZnO)-PDA interface and evaluated the influence of PDA on some optical properties of formed ZnO-PDA composite
Summary
Experimental sodium sulfate, zinc nitrate hexahydrate, and phosphate-buffered saline (tablet) were pur2.1. Aesar (PoZinc acetate dehydrate, hexamethylenetetramine, 2-propanol (IPA), ethanolamine, land), andsulfate, were used further purification. Glucose oxidasesaline was purified sodium zincwithout nitrateany hexahydrate, and phosphate-buffered (tablet)from were. Germany), 280 U/mg material), and purchased from purchased niger, from Sigma. (C and tris(hydroxymethyl)aminomethane 99% (A18494) were purchased from Alfa Aesar from. 15 min was performed purchased (Poland).in order to eliminate organic traces. The ITO glass substrates cleanednanostructures by successive sonication with deionized water electron microscope (SEM). 10 min,Evo with proper drying final use.Germany)), Oxygen plasma treatment for min was performed in order to eliminate organic traces. (Jena, and spectroscopy (EELS) detector and FTIR wasZeiss performed usingGermany)), an FTIR-ATR high-resolution transmission electron microscope (HR-TEM)MA,. ZnO nanorods (ZnO-NRs) were formed by the hydrothermal method (Scheme 1A)
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