Application of neem waste vermicompost in compensating nematode induced stress and upregulating physiological markers of tomato plants under glass house conditions after 10 days of exposure.

Abstract

Farming, food processing, animal husbandry and other agro-based activities contribute to global environmental degradation by producing millions of tons of organic and inorganic solid waste. In terms of sustainable agriculture, agricultural waste management and conversion into useful products are essential. In addition, plants are facing various kinds of biotic stress, which ultimately affects their defense system. Altered defense systems in plants ultimately lead to the death of plants and a reduction in crop production. The present study is designed to keep the abovementioned fact in mind, which mainly focuses on the reuse of agricultural waste and its application to the antioxidant potential and structural components of tomato plants during nematode stress. In the present study, neem leaves were collected and mixed with cattle dung for the preparation of vermicompost. Then, tomato seeds were pre-treated with vermicompost extract before being germinated in earthen pots. After germination, they were transplanted to separate pots and inoculated with freshly hatched juveniles of Meloidogyne incognita. The experiments were conducted for 10 days under glass house conditions, and after that, plants were harvested and various physiological (antioxidant capacity, percent electrolyte leakage) and structural markers (carbohydrate content, Fourier transform infrared spectroscopy) were analyzed. Results revealed that all physico-chemical properties make vermicompost superior as compared to soil and pre-compost material. Further, nematode stress leads to altered physiological and structural markers as compared to uninfected seedlings. However, treatment with vermicompost significantly increases carbohydrate content and antioxidative capacity in a concentration dependent manner. In addition, electrolyte leakage was found to be decrease with an increase in the concentration of vermicompost. All these findings conclude that vermicompost has strong potential to limit the damage caused by nematodes and boost the antioxidant potential of the host plants. Further, this study provides strong evidence for using vermicompost as an eco-friendly alternative to chemical nematicides and a potential strategy for agricultural waste management. This is the first study in which the tomato plant's structural and physiological markers were assessed during nematode stress after being supplemented with vermicompost under glass house conditions for an initial 10 days of nematode exposure.