Abstract
The combination of nanodiamond (ND) with biomolecular mass spectrometry (MS) makes rapid, sensitive detection of biopolymers from complex biosamples feasible. Due to its chemical inertness, optical transparency and biocompatibility, the advantage of NDs in MS study is unique. Furthermore, functionalization on the surfaces of NDs expands their application in the fields of proteomics and genomics for specific requirements greatly. This review presents methods of MS analysis based on solid phase extraction and elution on NDs and different application examples including peptide, protein, DNA, glycan and others. Owing to the quick development of nanotechnology, surface chemistry, new MS methods and the intense interest in proteomics and genomics, a huge increase of their applications in biomolecular MS analysis in the near future can be predicted.
Highlights
As a product of the detonation of carbon-based explosives, nanodiamond (ND) was firstly formed in the 1960s by Russian researchers [1]
An effective and rapid way to remove the proteins and peptides form the digestion solutions of glycoproteins is very important for improving matrixassisted laser desorption/ionization (MALDI)-mass spectrometry (MS) analysis of neutral underivatized glycans released from glycoproteins
ND can be readily used as a solid phase extraction (SPE) material for enriching peptides and proteins for direct MALDI-MS
Summary
As a product of the detonation of carbon-based explosives, nanodiamond (ND) was firstly formed in the 1960s by Russian researchers [1]. Due to its low cytotoxicity and no sign of photobleaching, this material has become a very important fluorescent probe for intracellular processes and has promise in drug delivery for cancer therapeutics [7,8,9,10]. As such a rediscovered material, ND and its applications have recently been prospected and reviewed by several researchers [3,4,5,6]. Most examples in this review will show the application of NDs in the MALDI-MS, but some applications based on ESI-MS will be discussed too
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
