Abstract
A chitosan film containing MnO 2 nanoparticles was introduced to amperometric glucose biosensors for the elimination of the interference from ascorbic acid. Chitosan was mixed with MnO 2 nanoparticles and electrochemically deposited on electrodes previously modified with glucose oxidase. MnO 2 nanoparticles incorporated in the chitosan film was very stable and can effectively oxidize AA to electrochemical inactive product, and thus eliminate the interference of AA on the glucose biosensors based on the direct oxidation of hydrogen peroxide. The effect of the content of MnO 2 nanoparticles and the deposition time on the characteristics of the film, as well as the stability of the film during continuous exposure to AA was investigated. Glucose biosensors deposited with the film containing MnO 2 nanoparticles were suitable for determination of glucose in the presence of AA.
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