Abstract
Spectral luminescent analysis of single cells and cellular systems enable us to reveal the initial changes of intracellular metabolism that can followed by human diseases or failure in biocenosis. Two cytodiagnostic systems of de-vices and techniques have been developed: 1) Microspectrofluorimeters registering the fluo-rescent spectra of individual cells or intracellular organelles used for fundamental investigations of cell reactions and for discovering and studying new dimensionless fluorescent characteristic parameters reflecting the biochemical or physiological properties of the cells; 2) Double- and multi-wave microfluorimeters for rapid registration of fluorescent characteristic para- meters for many cells to obtain statistical information about cell population. These techni- ques are useful especially in medical and eco-logical investigations.
Highlights
All cells have a general physical-chemical basis of functioning in spite of different morphological structures and functions
Karnaukhova et al / Natural Science 2 (2010) 444-449 rimeters registering the fluorescent spectra of individual cells or intracellular organelles are used for fundamental investigations of cell reactions and for discovering and studying of the new dimensionless fluorescent characteristic parameters, reflecting the biological or physiological properties of the cells
Of particular importance may be the application of the parameters φ and ψ for predicting the initiation of the disease “ciguatera”. It is the poisoning of people with usually harmless fishes and mollusks living in regions of intense “blooming” of blue-green algae in tropic zones of the oceans [19]. These few examples indicate that the methods of luminescent cytodiagnostics have promising applications in different fields of biology, medicine, environment protection and biotechnology
Summary
All cells have a general physical-chemical basis of functioning in spite of different morphological structures and functions (synthesis of nucleic acids and proteins, energetic, etc.). The characteristic features common for cells of different kinds were defined on the base of fundamental studies of intracellular regulation of metabolism with microspectral luminescent analysis. It formed a new trend in automation of cytodiagnostics. The advance in fluorescent cytodiagnostics is only possible with best methods and tools available for microspectral analysis which present a combination of a fluorescent microscope with a spectroanalysing instrument supplied with electronic registration and control units. Such a system satisfying all needs of cytodiagnostics would consist of the two types of instruments. The interconnection between them is determined by their functional peculiarities which will be considered below with some characteristic parameters using in medicine and ecology [1,2,3]
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