Abstract
Concanavalin A layered calcium alginate-cellulose beads adsorbed and cross-linked peroxidase of Momordica charantia was employed for the treatment of p-bromophenol polluted water. Immobilized peroxidase showed remarkably higher storage stability and retained about 78% phenolic compound removal efficiency over a period of one-month's storage at 4 °C. After a fourth repeated use immobilized enzyme retained nearly 50% p-bromophenol removal efficiency. p-Bromophenol removal by immobilized enzyme was ∼84% in the presence of 0.1 mM HgCl2. A significantly higher concentration of p-bromophenol was removed by immobilized enzyme in the presence of water-miscible organic solvents as compared to free enzyme. In stirred batch processes nearly 91%, 94% and 83% of p-bromophenol was removed in 3 h at 30, 40 and 50 °C, respectively. Immobilized enzyme present in two different reactors and operated at flow rates of 10 and 20 ml h−1 retained 75 and 65% p-bromophenol removal efficiency even after one month of their continuous operation. Absorption spectra for treated and untreated p-bromophenol exhibited a marked difference in absorbance at various wavelengths. Hence, it is concluded that reactors filled with immobilized enzymes can successfully be operated for the treatment of huge volumes of effluent containing various types of aromatic pollutants.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
