Abstract
A chromatographic method utilizing a carboxymethylated poly(vinyl alcohol) resin for a more accurate determination of stable haemoglobin A 1c (St-A 1c) has been developed. The complete separation between St-A 1c, labile HbA 1c (L-A 1c) and HbF was achieved by gradient elution with sodium chloride in phosphate buffer. This high resolution permits accurate quantitation of St-A 1c, even in the presence of high levels of HbF or L-A 1c. In 142 subjects with normal fasting plasma glucose and normal response to a 75-g oral glucose tolerance test, the reference interval of St-A 1c was 2.80–3.98%.
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