Abstract
Residues from the hot aqueous ethanol extracts of twigs were fractionated to obtain water-soluble fractions which were subsequently deionized, and then methylated by a modification of Kuhn's procedure. Gas-liquid partition chromatography (GLPC) was used for the qualitative analysis of the products of methylation, and to isolate individual constituents. The identities of the latter were established or confirmed by infrared and proton magnetic resonance (PMR) studies. This general procedure appears to be useful for chemo-taxonomical surveys, for the detection of new constituents, and for biosynthetic studies using tracer techniques. When the method was applied to twigs of Picea mariana (Mill.) BSP., Picea glauca (Moench) Voss and Taxus sp., monosaccharides, sucrose, and raffinose were detected readily. P. glauca contained in addition both picein and pungenin, whereas P. mariana did not contain these glucosides. PMR studies revealed that the acetyl groups in picein and pungenin were converted to isobutyryl groups, in quantitative yield, during the methylation procedure. Taxicatin was found in Taxus together with several unidentified constituents.
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