Application of Dual-Drug Loaded Metal Organic Framework Nanomaterials Targeting PI3K Signaling Pathway in Triple Negative Breast Cancer Cells

Abstract

Aim: To design a new treatment based AKT inhibitor (MLF), as a therapeutic target, and doxorubicin, as a chemotherapy agent, co-loaded on Modern metal organic frameworks (MFOs) by using ZIF-8 nanomaterials. Methods: We synthesized a MOFs (ZIF-8) as a drug carrier, and achieved simultaneous loading of the chemotherapeutic drug DOX and AKT inhibitor MFL (ZIF-8/DOX/MFL), as a drug carrier. In addition, we used MTT assay, evaluation the expression of collagen-1, and tumor drug uptake to evaluate the efficacy of our treatment. We further used ultrasound to modify the red blood cell membrane on the surface of ZIF-8/DOX/MLF to improve the biological safety and stability of the drug-carrying system, and finally obtained ZIF-8/DOX/MLF/RBCM. Results: Our results showed that the tumor enrichment in the ZIF-8/MLF/RBCM group was about 3 times that of the ZIF-8/RBCM group; and both in vivo and in vitro imaging of mouse organs showed that MLF has the function of assisting drug delivery and enhancing drug tumor enrichment, according to fluorescence quantification. Conclusion: The research results show that the ZIF-8/DOX/MLF/RBCM can achieve the purpose of sensitizing new tumor chemotherapy in breast cancer.