Abstract
Recently, enzymatic treatment using peroxidases in removal of aromatic compounds has gained importance. In this study pointed gourd peroxidase was salt fractionated and direct immobilization of these proteins on diethylaminoethyl cellulose for oxidation of phenol and α-naphthol has been investigated. The activated diethylaminoethyl cellulose was quite effective in high yield immobilization of peroxidases from pointed gourd and it could bind ~576 units per g of the matrix. Immobilized pointed gourd peroxidase on this anion exchanger showed very high effectiveness factor ‘Ɛ’ as 0.91 with an activity yield of 91%. Immobilized PGP (I-PGP) as compared to soluble counterparts (s-PGP) were more effective and removed 79%, 88% and 54% oxidation of phenol and α-naphthol by 75%, 81% and 61% at 30, 40 and 50°C respectively, with a treatment time of 140 min. In the absence CdCl2 s-PGP as well as I-PGP exhibited upto 93% of oxidation of these compounds; whereas the presence of CdCl2 of negatively affected the removal of phenol and α-naphthol. The reactor worked well continuously for over one month for effectively oxidizing/ removing phenol and α-naphthol by 54% and 61% respectively. Thus, such immobilized enzyme systems in reactor have a great future and could be exploited for treating organic pollutants present in industrial effluents.
Highlights
The presence of different aromatic compounds in wastewater from several industrial effluents is of great environmental concern
Most phenolic compounds are toxic, pose health risk and have been classified as hazardous pollutants that can accumulate in the food chain [2,3,4]. α-Naphthol is a toxic hydroxylated metabolite of polycyclic aromatic hydrocarbon naphthalene widely used in the manufacture of plastics, rubber, synthetic fibers and dyes
Under similar conditions of treatment Immobilized pointed gourd peroxidase (PGP) (I-PGP) catalyzed the oxidative removal of α-naphthol by 75%, 81% and 61% at respective temperatures of 30, 40 and 50°C
Summary
The presence of different aromatic compounds in wastewater from several industrial effluents is of great environmental concern. Phenolic compounds and its derivatives have their sources from a number of chemical industries such as petroleum, coal conversion, resins, textile dyes and paper processing [1]. Α-Naphthol is a toxic hydroxylated metabolite of polycyclic aromatic hydrocarbon naphthalene widely used in the manufacture of plastics, rubber, synthetic fibers and dyes [5,6,7]. Due to their potential lethality, wastewater must be treated for the removal of these compounds/derivates prior to its final discharge into environment. A number of conventional strategies are available for treating these aromatic compounds which includes microbial degradation, solvent extraction, chemical oxidation, incineration, adsorption on activated charcoal and etc. These methods are effective in removing the pollutants with limitations of being expensive, time consuming, and low efficiency, applicability to a limited concentration range and formation of soluble toxic by-products [13,14,15]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
