Application of CTAB-Coated Magnetic Nanoparticles for Solid-Phase Extraction of Thiamine Hydrochloride from Pharmaceutical Formulations and Urine Samples

Abstract

This study investigated an efficient and low-cost magnetic solid-phase microextraction engaged with spectrophotometry method for extraction and determination of thiamine hydrochloride (THY) in pharmaceutical and urine samples. Adsorption and desorption of red azo dye findings from THY coupling with the diazotized drug compound (sulfadimidine sodium) in an alkaline medium were established by magnetic iron oxide nanoparticles (MNPs) coated with cetyltrimethylammonium bromide (CTAB). The extracted azo dye product was measured spectrophotometrically at λmax of 510 nm. The azo dye removal efficiency of modified MNPs was assessed by investigating several conditions, such as the concentration of reagents, surfactant amount, adsorbent amount, pH of the solution, contact time, sample volume, and desorption factors. Under the optimized conditions, the removal percentage of the azo dye of 98% with good linearity over the investigated concentrations range 0.1–5 μg/mL was obtained. Limits of detection and quantification were 0.053 and 0.18 μg/mL, respectively, with the relative standard deviation (RSD) better than 3%. The method was applied to estimate THY in different matrices using the CTAB-coated MNPs, within an extraction time of 10 min.