Abstract
The human amniotic membrane has been widely used in clinical practice to treat all kinds of lesions related to the epithelial surface, corneal epithelial reconstruction, and orthopaedic trauma. Currently, another application aspect of the amniotic membrane of great interest is its use as an in vitro adherent cell culture medium. In this study, the human amniotic membranes were collected under sterile conditions in the operating room and tested negative for HIV, HBV, HCV, and VDRL. The amniotic membranes were then processed to remove the epithelial layer, to obtain a collagen membrane that can be used as a substrate for cell culture adhesion. Following that, the collagen membrane was structurally evaluated by Hematoxylin and eosin (H&E) staining, SEM. Next, we tested the use of collagen membranes as a culture medium for human fibroblasts and evaluated cell adhesion, growth, and development by histological images over time of cell culture. The image results obtained before and after processing showed that the collagen membrane has an acellular structure, maintaining the basement membrane’s structure. Cell culture results exhibited that fibroblasts adhered and developed well on this collagen membrane.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.