Application of chromosome-specific monoclonal antibodies in wheat genetics

Abstract

A monoclonal antibody (P24B) to a wheat gliadin protein coded by a gene on the short arm of chromosome 1B was used as a chromosome marker. Somatic chromosome number for the 1B chromosome, as predicted by the level of binding of the antibody to extracts from seeds of single cross F2 and testcross F1 populations, was confirmed with sodium dodecyl sulfate – polyacrylamide gel electrophoresis and root-tip analysis. The implications of monoclonal antibodies as tools for cytogenetic analysis are discussed.Key words: polyacrylamide gel electrophoresis, aneuploid, cytogenetics, seed storage proteins.