Application of CF effect, IAR and RAPD methods in evaluation or competitiveness between inoculated and indigenous strains of Rhizobium leguminosarum bv. trifolii

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The paper evaluates the competitive ability of Rhizobium leguminosarum bv trifolii strain T49B1 used as inoculant for Trifolium repens L. (Viola) and indigenous population of rhizobia from chernozem, stagnosol and humic gleysol. Nodule bacteria were identified using calcofluor effects (CF) based on succinoglycan production, and confirmed by IAR patterns and RAPD fingerprinting (using SPH1 primer). T49B1 nodules occupancy level from 71.78% in pseudoglay to 89.56% in chernozem was detected. Inoculation by T49B1 strain showed it to be a very good competitor with the possibility to establish effective symbiosis and increase protein content compared to non-inoculated (control) plants.