Abstract
Agricultural crops have been increasingly attacked by microorganisms that cause low productivity and severe losses to producers. In this context, chemical products are no longer sufficient to control pathogens, leading to the introduction of biological inoculants that can inhibit unwanted microorganisms. However, these products must be formulated properly to have their useful life guaranteed. In this sense, this study aimed to assess the use of carboxymethylcellulose and xanthan gum biopolymers in the conservation of cells of Pseudomonas fluorencens, an effective rhizobacteria in the biological control of the Macrophomina sp. fungus, responsible for black rot in different cultures. The inoculant produced from the bacteria fermentation was stored and assessed at 7, 15, 30, 45, 60, 90, 120, 150, 180, and 210 days after adding the biopolymers. The cell culture was then fermented and added with biopolymers, followed by pH measure and CFU mL-1 count. Antagonist activity against Macrophomina sp. Was assessed through the methods of fungal culture method in the antagonist culture, paired culture, and central risk pairing. Assessments were performed at 24, 48, and 72 hours after incubation in a growth chamber. Percentage of growth inhibition was then calculated and subjected to Tukey test at 5% significance level. According to the results, the biopolymers were able to preserve the Pseudomonas fluorescens cells for 210 days and ensure better antagonist potential against the pathogen, corroborating that the market should provide products with longer shelf life
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call