Abstract
The paper presents the results of studying biological methods of increasing the yield of planting material for grapes and increasing the germination of hybrid seeds to accelerate the selection process. The impact of bioactive compounds (6-benzylaminopurine and gibberellic acid) in culture medium on production of grape clones during micropropagation stage was studied. Modified culture medium of the A.N. Rebrov’s patent was determined as having the most potential on the first stage of grape explant cultivation. The establishment of grape regenerant plants was up to 80%, depending on the variety. On the second and third passages Rebrov’s culture medium was modified by the addition of 6-benzylaminopurine in the concentration 0.35 mg/l and gibberellic acid in the concentration 0.1 mg/l. The method of prestratification soaking of seedlings seeds in the solutions of gibberellic acid of various concentrations was also studied to accelerate the breeding process. The production of seedlings increased to 91.2% at the concentration of gibberellic acid 0.01% that was higher than control production on 31.2%.
Highlights
Nowadays the development of viticulture is one of the priority areas in the system of developing plans and programs for strategic planning of industry in the Russian Federation. [1]
The production of seedlings increased to 91.2% at the concentration of gibberellic acid 0.01% that was higher than control production on 31.2%
The low reproduction rate is the main deterrent to the process of introducing a new variety into production using traditional methods, due to the inability to obtain a large amount of grape planting material in one season [6]
Summary
Nowadays the development of viticulture is one of the priority areas in the system of developing plans and programs for strategic planning of industry in the Russian Federation. [1]. The intensification of viticulture determines the need to develop new effective technologies, directions and methods, their inclusion in the production system of healthy planting material [5]. The low reproduction rate is the main deterrent to the process of introducing a new variety into production using traditional methods, due to the inability to obtain a large amount of grape planting material in one season [6]. The application of bioactive compounds in pre-stratification soaking of grape seeds can be used to increase the germination of hybrid seeds and accelerating the process of breeding new grape varieties, consequentially [9, 10]. The study of ways to optimize the nutrient medium to increase the multiplication factor in vitro and, increase the efficiency of the process of obtaining a healthy planting material for grapes is a very relevant area of research
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