Abstract
A whole-blood lymphocyte stimulation test (WBLST), the standard plate and tube agglutination tests, the Brucella buffered antigen test, the 2-mercaptoethanol agglutination test, the Rivanol plate precipitation test and bacteriological isolation were utilized in a brucellosis outbreak investigation in a beef herd. Three of the animals were classified as not infected serologically. However, these 3 animals were classified as infected on the WBLST, andBrucella abortus biotype 1 (not strain 19) was isolated from their lymph nodes. The WBLST exhibited significant sensitivity in this investigation and more observations of this nature might strengthen the application of this assay in similar situations.
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