Abstract
Background and Objectives: To quantify the cellular isoform of prion protein (PrP<sup>c</sup>) in human blood using a new time–resolved dissociation–enhanced fluoroimmunoassay (DELFIA<sup>®</sup>). Materials and Methods: The DELFIA was optimised for human blood samples and applied to isolated cell and plasma fractions from blood donations. The physicochemical properties of PrP<sup>c</sup> were analysed. Results: 26.5% of blood PrP<sup>c</sup> was associated with the platelet fraction, 0.8% with polymorphonuclear leucocytes, 2.4% with mononuclear leucocytes, 1.8% with red cells and 68.5% with plasma (mean values from 4 processed donations). Conclusion: The majority of blood PrP<sup>c</sup> is found in the platelet and plasma compartments.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call