Application of a New Type of Natural Calcined Bone Repair Material Combined with Concentrated Growth Factors in Bone Regeneration in Rabbit Critical-Sized Calvarial Defect.

Zhenxing Liu,Xiaoyang Wang,Shuqing Tong,Shengyun Huang,Dongsheng Zhang,Li Ma,Roberto Martínez-Beamonte

doi:10.1155/2020/8810747

Zhenxing Liu, Xiaoyang Wang + Show 5 more

Open Access

https://doi.org/10.1155/2020/8810747

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Journal: BioMed research international	Publication Date: Nov 24, 2020
Citations: 5	License type: CC BY 4.0

Affiliation: Shandong Provincial Hospital

Abstract

Purpose This study is aimed at investigating bone regeneration in critical-sized defects in rabbit calvarium using a novel nano- (n-) hydroxyapatite hybrid scaffold with concentrated growth factors (CGFs). Methods Twenty-four male adult rabbits were chosen to establish a critical-sized bone defect model and randomly divided into two groups. Two defects of 15 mm diameter each were created in the parietal bone of each animal. Group A had n-hydroxyapatite hybrid scaffold placed in the experimental defect on the right, and the left defect was unfilled as blank. Group B had hydroxyapatite hybrid scaffold mixed with CGF placed in the right defect and CGF on the left. Six animals in each group were sacrificed after 6 and 12 weeks. Cone-beam computed tomography system scanning and hematoxylin and eosin (HE) staining were used to detect osteogenesis within the defects. Results The treatment with n-hydroxyapatite hybrid scaffold along with CGF resulted in a significantly higher amount of new bone at 6 and 12 weeks compared to the treatment with CGF alone and the controls. No apparent inflammation and foreign body reaction were observed through HE staining. Conclusions The new synthesized n-hydroxyapatite hybrid scaffold and CGF can be applied for bone defect regeneration to promote the process to a certain extent.

Highlights

The regeneration of bone defects in the maxillofacial skeleton is still challenging in the clinic, especially large defects in trauma, osteotomy surgery, oncologic resections, developmental anomalies, or socket preservation before implantation, in which defects need to be reconstructed [1, 2]
When operation sites were reexposed after sacrifice, both groups showed new bone formation at the defects on each side
Cone-Beam Computed Tomography (CBCT) results showed that the bone density of the right defects of all animals, both in Group A and Group B, 6 and 12 weeks after the operation, was much higher than that of the left defects

Summary

Introduction

The regeneration of bone defects in the maxillofacial skeleton is still challenging in the clinic, especially large defects in trauma, osteotomy surgery, oncologic resections, developmental anomalies, or socket preservation before implantation, in which defects need to be reconstructed [1, 2]. Autogenous bone grafts are the best material for defect reconstruction, as there is no rejection. Problems remain as the grafts may be insufficient for large defects, which may lead to additional surgical trauma and significant resorption [3]. Hydroxyapatite bone substitutes have been widely used in the clinic over the years [4,5,6]. They can be obtained in many ways, making them immunologically harmless. They stimulate osteogenesis, incorporate into the defects, and provide a scaffold for the ingrowth of vessels and cells to facilitate new bone formation.

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