Abstract
Based on a simple model, the influence of the experimental parameters on solvent strength and selectivity in reversed-phase ion-pair high-performance liquid chromatography is discussed. For optimization of the separation, a mixture design is proposed in which the pH, the content of organic modifier and the concentration of the pairing ion in the mobile phase vary. This design was applied to the separation of two mixtures of four pharmaceutically important amines (water-soluble vitamins and local anaesthetics). By a suitable choice of the three (pseudo) components, which are mixed to compose the mobile phase, it is possible to optimize the resolution of the worst separated pair of peaks and to locate in the optimal region the chromatogram with the minimal analysis time.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
