Abstract
Sixteen phenylamine- and naphthylaminesulphonic acids which are negatively charged and weakly retained or non-retained in reversed-phase high-performance liquid chromatography were used as model compounds to examine the quantitatively relationship between the calculated n-octanol-water partition coefficient (log P) and the retention value (log k′) (or log k w) and solute charges in reversed-phase ion-pair liquid chromatography (RP-IPC). It was observed that the correlation of log P vs. log k′ (or log k w) for solutes with one negative charge has moderate regression coefficients of ca. 0.80, but the correlation of log P vs. log k′ (log k w) and solute charges ( n e) for solutes with different charges has a regression coefficient higher than 0.98. The log k′ (or log k w) value in RP-IPC always make a positive contribution and the solute charges always makes a negative contribution to log P values. The proposed relationship between the log P value and tje log k′ value and solute charges makes it possible to predict the n-octanol-water log P values of ionic compounds from the retention values in RP-IPC, and is also useful in elucidating the retention mechanism in RP-IPC.
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