Application of a Conducting Poly-Methionine/Gold Nanoparticles-Modified Sensor for the Electrochemical Detection of Paroxetine.

Saedah R Al-Mhyawi,Riham K Ahmed,Rasha M El Nashar

doi:10.3390/polym13223981

Saedah R Al-Mhyawi, Riham K Ahmed + Show 1 more

Open Access

https://doi.org/10.3390/polym13223981

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Journal: Polymers	Publication Date: Nov 17, 2021
Citations: 8	License type: CC BY 4.0

Affiliation: Jeddah University, Cairo University

Abstract

This work demonstrates a facile electropolymerization of a dl-methionine (dl-met) conducting polymeric film on a gold nanoparticle (AuNPs)-modified glassy carbon electrode (GCE). The resulting sensor was successfully applied for the sensitive detection of paroxetine·HCl (PRX), a selective serotonin (5-HT) reuptake inhibitor (SSRIs), in its pharmaceutical formulations. The sensor was characterized morphologically using scanning electron microscopy (SEM) with energy dispersive X-ray spectroscopy (EDX) and atomic force microscopy (AFM) and electrochemical techniques such as differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The proposed sensor, poly (dl-met)/AuNPs-GCE, exhibited a linear response range from 5 × 10−11 to 5 × 10−8 M and from 5 × 10−8 to 1 × 10−4 M using DPV with lowest limit of detection (LOD = 1 × 10−11 M) based on (S/N = 3). The poly (dl-met)/AuNPs-GCE sensor was successfully applied for PRX determination in three different pharmaceutical formulations with percent recoveries between 96.29% and 103.40% ± SD (±0.02 and ±0.58, respectively).

Highlights

Paroxetine hydrochloride hemihydrate (PRX, (3S,4R)-4-(4-fluorophenyl)-3-(3, 4-methylenedioxyphenoxymethyl) piperidine) is known as a selective serotonin (5-HT) reuptake inhibitor (SSRIs) antidepressant drug, used to treat depression, panic disorders, generalized and social anxiety disorders, phobias, posttraumatic stress disorders and obsessive compulsive disorders [1]
PRX inhibits the noradrenaline transporter from doing its function, allowing noradrenaline to persist in the synapse for longer rendering normal levels of noradrenaline to be reached in humans [2,4,5]
Effect of Deposition Time of AuNPs Before electropolymerization of DL-met, the surface of glassy carbon electrode (GCE) was modified at different deposition time intervals with AuNPs, and the IPRX of 10 μM PRX on the AuNPs-GCE were recorded and compared with the response of the bare GCE

Summary

Introduction

Paroxetine hydrochloride hemihydrate (PRX, (3S,4R)-4-(4-fluorophenyl)-3-(3, 4-methylenedioxyphenoxymethyl) piperidine) is known as a selective serotonin (5-HT) reuptake inhibitor (SSRIs) antidepressant drug, used to treat depression, panic disorders, generalized and social anxiety disorders, phobias, posttraumatic stress disorders and obsessive compulsive disorders [1]. Clinical effectiveness, and favorable side effect profile, after fluoxetine, PRX has become the second most often prescribed SSRI antidepressant [2,3]. This drug is believed to have therapeutic effects on the brain by working as a highly selective inhibitor of the serotonin transporter (SERT) and norepinephrine transporter (NET). PRX inhibits the noradrenaline transporter from doing its function, allowing noradrenaline to persist in the synapse for longer rendering normal levels of noradrenaline to be reached in humans [2,4,5]

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