Abstract
Objective. To explore the application of 3D image technology in motor and sensory nerve classification. Methods. A total of 200 sections of the 5cm-long popliteal fossa peroneal nerve from adult volunteers were cut and frozen. The slices were 10 m thick, and the interval between the slices was 0.25 mm. Acetylcholinesterase histochemical staining was used to observe the changes of nerve bundles under the microscope. The stained sections were transformed into digital images by the digital camera system, and the images were stitched to obtain a two-dimensional panoramic image 100 times magnified. The properties of the functional bundles were manually judged. Using Amira 3.1 three‐dimensional reconstruction software to realize the three-dimensional reconstruction and visualization of nerve can not only accurately perceive the complex three-dimensional surface structure of nerve, but also arbitrarily display, rotate, scale, and segment the three-dimensional structure inside nerve, and carry out three-dimensional measurement in time. It has made preliminary achievements in brachial plexus, lumbosacral plexus, neural stem functional bundle (group), and intramuscular nerve routing and distribution, including the regeneration process of sensory nerve and three-dimensional reconstruction and visualization of composite tissue containing sensory nerve. Conclusion. Based on histology and computer technology, the functional band of short peroneal nerve can be reconstructed in 3D, which provides a feasible basis for the three-dimensional reconstruction of the functional band of the long peripheral nerve.
Highlights
3D image reconstruction using 2D data sets of biological tissue has been vigorously developed in many fields, including 3D reconstruction of CT and MRI images in medicine, laser confocal reconstruction of biological tissue microstructure, and 3D visual reconstruction of scanning electron microscopy. 3D reconstruction technology has been widely used in the field of medicine and plays an increasingly important role in the development of medicine
Two-dimensional images of tissue sections were obtained by using the optical microscope
Due to a series of difficulties in positioning, image distortion, and image segmentation, 3D reconstruction technology still lags behind the development of CT, MRI, and 3D laser scanning confocal microscope
Summary
This three-dimensional reconstruction is time-consuming and imprecise. Due to the development of computer graphics, a series of two-dimensional images can be analyzed, recognized, and processed to establish a three-dimensional image. Due to the development of computer software and hardware, the ability of using computer to reconstruct three-dimensional images of biological specimens has been rapidly developed in the past ten years. 3D reconstruction technology has been widely used in the field of medicine and plays an increasingly important role in the development of medicine. In recent years, developed countries have made use of scientific computing visualization technology to visually display the internal structure of the human body. In recent years, developed countries have made use of scientific computing visualization technology to visually display the internal structure of the human body. e most typical example is the “Visible Man” project in the United States
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