Abstract
The essential oil from leaves and stems of clove (Syzigium Aromaticum) growing in Siwa (South Sulawesi, Indonesia), was obtained by hydrodistillation and gas chromatography-mass spectrometry (GC-MS) was used to identify the constituent of the sample. There were five most compounds bioactive contained in the clove oil of the leaves and stems, namely eugenol, b-caryophyllene, 1,4,7- cycloundecatriene-1,5,9,9 tetramethyl, caryophyllene oxide, and cadinene. The eugenol compound, which is contained in the stems is 55.83% bigger than the leaves, which is 45.3%. But on the contrary, other compounds in the leaves are b-caryophyllene (25.57%), 1,4,7- cycloundecatriene-1,5,9,9 tetramethyl (8.65%), caryophyllene oxide (4.64%) and cadinene (2.31%) greater than the other compounds in the stems, namely b-caryophyllene (19.72%), 1,4,7- cycloundecatriene-1,5,9,9 tetramethyl (4.38%), caryophyllene oxide (3.48%) and cadinene (0.5%). Eugenol is the major constituent of the clove oil that can be used in pharmacology as antivirus, antimicrobial, antiseptic, and industry of perfume.
Highlights
Clove plant is one of the plant's species of vegetation that produces essential oils known as clove oil
gas chromatography-mass spectrometry (GC-MS) Analysis The GC-MS analysis achieved component identification. 1 mL of extract clove oil was dissolved in acetone and placed in a vial
The oven temperature of GC-MS was programmed commencing from 70 °C with a hold time of 2 min, and the temperature was increased to 200 °C at a rate of 10 °C/min and the final temperature was 280°C with a holding time of 9 min at a rate of 10 °C/min so that the total time of analysis was 36 min
Summary
Clove plant is one of the plant's species of vegetation that produces essential oils known as clove oil. Essential extracts obtained were widely applied in the field of pharmacology. Clove oil gives a distinctive aroma of essential originating from flowers (10-20%), stems (5-10%), and a small portion of the leaves [12]. Clove oil contains three large compositions, namely eugenol (70-85%), eugenyl acetate (15%), -caryophyllene (5-12%), and a small number of other bioactive compounds [12]. Gas chromatography-mass spectrometer (GC-MS) is the most popular method for the determination of essential oil composition. The work started here, aiming to fill the blank field through the analysis constituent of essential oil from extracted oil from leaves and stems of clove. GC-MS was employed to identify compounds in the extracted oil of sample, and It would reveal how many concentrations contained in the sample
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