Abstract
Objective To discuss the effect of chronic restraint stress(CRS)on gastroesophageal histological changes in mice for investigating the role of psychological factor in the pathogenesis of gastroesophageal reflux disease, and provide with referential parameters for successful establishment of CRS model. Methods 20male KunMing mice were randomly divided into two groups which are CRS and normal control(NC)group.Mice in CRS group were submitted to partial restraint stress for 2 h per day.After 14 days of restraint stress, glucose tolerance test(GTT)and insulin tolerance test(ITT)were performed firstly, and esophagus, stomach and blood sample of all mice were taken for further detecting the parameters and analyzing histological changes in tissues through HE staining. Results Body weight increment of mice in CRS group is significantly lower than that of NC group(P<0.05). ITT shows that CRS group happened insulin resistance.The result of ELISA indicated that the level of hormones related to HPA axis in CRS group was significantly higher than those of NC mice(P<0.05). Histological changes appeared in CRS group include hyperemia, edema and infiltration of inflammatory cells in gastroesophageal mucosa. Conclusion CRS obviously affects physiological activities and may result in GERD-related histopathological changes in stomach and esophagus.Our study provides with not only simple, but also effective experimental basis which has referential value on the research of investigating the role of psychological factor in the pathogenesis of GERD. Key words: Chronic restraint stress; Gastroesophageal reflux disease; Mice
Published Version
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