Application and comparison of proteolytic enzyme preparations in technology of protein hydrolyzates

Aleksandr Lukin

doi:10.1590/fst.09319

Abstract

Abstract Protepsin belongs to the class of acid proteinases, containing carboxyl groups in the active center. The preparation cleaves peptide bonds formed by aromatic amino acids (tyrosine, phenylalanine), and does not hydrolize esters and amides. Along with the proteolytic complex, Protepsin contains collagenase, lipase, and other digestive enzymes. The preparation is obtained according to the original technology through maceration of animal endocrine raw materials, followed by purification, concentration, isolation and freeze dehydration of the enzyme complex. The results of the laboratory studies of the proteolytic activity of enzyme preparations show that Protepsin, the preparation of animal origin with a proteolytic activity of 100 units/g, has the greatest collagenase activity in the biomodification of native collagen-containing raw materials.

Highlights

Nowadays food industry is among a large number of modern biotechnological industries the successful development of which is in no small way determined by the implementation of technology involving enzyme preparations
Proteolytic activity determines the degree of impact of any proteolytic enzymes on protein molecules, it was necessary to determine its level for the selected enzyme preparations
To prepare a protein hydrolyzate, the collagen-containing raw materials was washed with running water at 18-20 °С for 20 minutes and fleshed

Summary

Introduction

Nowadays food industry is among a large number of modern biotechnological industries the successful development of which is in no small way determined by the implementation of technology involving enzyme preparations. The creation and introduction of innovative technologies has greatly expanded the scope of application of enzyme preparations. Proteolytic enzyme preparations are of particular importance, since they are associated with the transformation of protein – the main component of human constructive metabolism. They constitute the largest segment of the world market of enzymes, and it is with their use that the search for new sources and forms of protein foods is carried out. Proteases catalyze the hydrolysis of peptide bonds and, along with their great practical significance, play an important role in metabolism, the study of which is an important fundamental task (Aylangan & Öztan, 2008; Vidal et al, 2018)

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Results

Conclusion