Abstract
Apple Snails is a snail-like animal. Being snail-like animals recognized as an edible aquatic animal and widely employed in traditional Chinese medicine, this study intends to reveal pharmacological potential of Apple Snails polysaccharide. Crude soluble polysaccharide of Apple Snails was first extracted using the hot water extraction and ethanol precipitation method. And the crude polysaccharide was purified successively using the Sevag method. After purification we found that and apple snails is rich in polysaccharide and the total polysaccharide content can reach to 68.4%. Using Fenton reactions, we found that antioxidant potential efficiency of apple snails polysaccharide is dose-dependent and can reach to 83.77% at the concentration of 5 mg/mL. Importantly, apple snails polysaccharide can efficiently inhibit lung cancer cell line A-549 proliferation in vitro and over 50% cell was killed in the dose of 200 mg/ml at 24 hours post treatment, implying that apple snails polysaccharide is a potential anti-tumor candidate.
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