Abstract
An apparatus for high-voltage thin-layer electrophoresis designed to operate at a load of 5.0 kV and 50 mA and characterized by simplicity of construction and operation is described. The analysis of the relative contribution and interdependence of the following factors as they affect the operation and resolution ability of this unit was made: current-voltage relationships, electrical field gradients, solvent transport, heat exchange, wick material, salts, buffer type, and concentration. The relative mobilities of the majority of amino acids in a pH 2.5 formic acid buffer and in a pH 10.2 ammonium buffer are sufficiently characteristic to provide a basis for tentative identification. The mobility of the amino acids exhibits a striking functional dependence on the ionic charge, molecular weight, type of hetero atoms in the side chain, and type and concentration of electrolytes employed. The resolution ability of this technique is equivalent or superior to that obtained by high-voltage paper electrophoresis.
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