Abstract
Recent research has discovered that using aspirin for a long time lowers the long-term risk of certain cancers, particularly colon cancer. However, the mechanism of anti-cancerous activity of aspirin against lung cancer is less studied. A molecular docking strategy was employed to identify the possible targets of aspirin while5-Fluorouracil (5-FU) was used as a positive control against lung cancer cell line A459. The In-silico analysis suggested that Caspase-3, Bax, andBcl-2could be potential targets for aspirin. The estimation of binding energies for these proteins resulted in -5.2, -5.8, and -5.7 Kcal/mol, respectively, which were better than 5FU (-4.8, -4.6, and -4.4, respectively).Trypan blues dye exclusion test exhibited a reduction in cell viability with the increase in Aspirin concentration. The IC50 values of Aspirin were calculated as 2.79 mM by MTT assay. The treatment of A459 cells with aspirin enhanced the levels of apoptotic genes at mRNA as well as at protein levels. The effect on the A549 lung cancer cell line, this study contributes to a better understanding of how Aspirin and 5-FU work in lung cancer.
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