Abstract
To study apoptosis of individual cellular components of the vascular wall of coronary arteries at different morphological stages of atherosclerosis. The study was performed on coronary arteries taken from 52 deceased patients with atherosclerosis and coronary heart disease at different stages of atherogenesis. For morphological study prepared paraffin sections, which were stained for morphological studies were prepared paraffin sections, which were stained with hematoxylin and eosin, by Van Gieson, Masson, on lipids with Sudan black B, according to Van Cossu. To determine apoptosis, TUNEL method used in paraffin sections. Apoptotic index (AI) was calculated by TUNEL-positive cells and the average inner shell coronary artery around the perimeter each with increasing microscopic 1000. Investigation showed significant apoptosis (p < 0.05) increase in AI smooth muscle, endothelial cells, macrophages in the coronary arteries affected by atherosclerosis compared to intact control group vascular segments significant reduction AI endothelial, smooth muscle cells and macrophages (p < 0.05) traced from the early stages of atherogenic disorders to atheromatosis. It is established that apoptosis of smooth muscle cells, macrophages and endothelial cells is the most intensive on early stages of atherosclerotic process. In process of progressing of atherosclerosis intensity and prevalence of apoptosis of coronary artery wall cells decreases, and processes of necrosis becomes predominant. Apoptosis of coronary artery wall cells is valuable in increasing the zones of atheromatosis, plaque destabilizations, and also increases the risk of thrombosis and ulcerations.
Highlights
Цель исследования: изучить апоптоз отдельных клеточных компонентов сосудистой стенки коронарных артерий на различных морфологических стадиях атеросклероза
The study was performed on coronary arteries taken from 52 deceased patients with atherosclerosis and coronary heart disease at different stages of atherogenesis
For morphological study prepared paraffin sections, which were stained for morphological studies were prepared paraffin sections, which were stained with hematoxylin and eosin, by Van Gieson, Masson, on lipids with Sudan black B, according to Van Cossu. .To determine apoptosis, TUNEL method used in paraffin sections
Summary
Апоптоз клеток стенки коронарных артерий как фактор развития и прогрессирования коронаросклероза. Для определения апоптоза использовали метод TUNEL на парафиновых срезах. Установлено, что апоптоз гладкомышечных клеток, макрофагов и эндотелиоцитов наиболее интенсивно протекает на ранних стадиях атеросклеротического процесса. Апоптоз клеток коронарных артерий способствует расширению зоны атероматоза, дестабилизации бляшки, увеличивает риск развития тромбозов и образования изъязвлений. Что причиной гибели клеток Исследование клеточного состава коронарных сосудов внутри атеросклеротической бляшки является прямое ток- проводили на увеличении 400 и 1000 на микропрепарасическое воздействие на них, к примеру, свободных ради- тах, окрашенных гематоксилином и эозином и TUNEL-. Индуцированный ЛПНП апоптоз ГМК коронарной артерии по всему периметру каждого микроусиливался цитокинами — фактором некроза опухоли α препарата при увеличении 1000 по следующей формуле:. Ям атеросклеротической бляшки выявляются макрофаги и ГМК, погибающие по механизму апоптоза [15]
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