Abstract
Rat lipoproteins were separated from each other and from unbound apoproteins by chromatography on 10% agarose gel. Fresh rat serum yielded two main lipoprotein peaks: Peak I corresponded mainly to very low density lipoproteins (VLDL) and peak II corresponded to high density lipoproteins (HDL). Recovery of total cholesterol and apolipoprotein A-I (A-I) in the two peaks was similar when fresh serum or lipoproteins of d < 1.21 g/ml were used, but recovery of the arginine-rich apolipoprotein (ARP) was reduced in lipoproteins of d < 1.21 g/ml. Most of this ARP was lost from the second peak (HDL), and this dissociated apoprotein was recovered in a third peak when the serum fraction of d > 1.21 g/ml was applied to the same column. Comparison of lipoproteins isolated by gel chromatography with those obtained by conventional flotation ultracentrifugation showed that ∼30% of ARP was lost from VLDL and ∼60% was lost from HDL during ultracentrifugation. The ratio of ARP to A-I fell progressively across the second chromatographic peak, indicating heterogeneity of HDL apoproteins as a function of particle size.
Published Version
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