Abstract
Deep venous thrombosis (DVT) is one of the major health problems worldwide. Apolipoprotein L domain containing 1 (APOLD1) was reported to be downregulated in DVT. The present study intended to investigate whether APOLD1 affects thrombus formation in a rat model of DVT. The rat model of DVT was established by inferior vena cava (IVC) stenosis. At 6, 12, 24 and 48 h after IVC stenosis, the gross IVC with thrombus was dissected and observed. Then, the rats were pre-injected with the lentiviral overexpression vector, APOLD1-LVs, 1 h before IVC stenosis, to evaluate the influence of APOLD1 on thrombosis in rats. The serum levels of D-dimer and TAT as well as the content of TF in IVC tissues were detected by ELISA. IVC stenosis resulted in thrombus formation in rats, increased serum levels of D-dimer and TAT, and decreased APOLD1 expression. APOLD1 overexpression inhibited in vivo thrombosis, reduced serum levels of D-dimer, and downregulated TF activity and level. APOLD1 overexpression also increased p-PI3K and p-Akt protein levels. APOLD1 suppresses thrombus formation in a rat model of DVT via downregulating TF expression by activating the PI3K/Akt pathway.
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