Aphrodisiac activity of vanari gutika formulated using milk obtained from jersey cow (Bos taurus), indigenous cow (Bos indicus) and buffalo (Bubalus bubalis)

Abstract

Erectile dysfunction (ED) is a condition characterized by the inability to develop or maintain erection of the penis. Ayurveda recommends vanari gutika, prepared from Mucuna pruriens as one of the products suggested for the management of sexual dysfunction. We were interested to validate and compare aphrodisiac potential of vanari gutika formulated, as per the principles of Ayurveda, using milk obtained from jersey cow (Bos taurus), indigenous cow (Bos indicus) and buffalo (Bubalus bubalis). In order to assess the aphrodisiac activity, Swiss Albino mice were used as experimental subjects (n = 6 animals per group) with oral administration of aforesaid three formulations of vanari gutika with different doses i.e., 8.56 mg/mL, 17.14 mg/mL and compared with standard sildenafil citrate (5 mg/kg) and vehicle (milk+ghee) for 7 consecutive days. Animals were observed for mating behavior. Hormonal analysis, sperm count and histopathological studies were carried out to confirm physiological changes. The study exhibit marked changes in sexual behavior. Vanari gutika formulated using milk from indigenous cow at higher dose (17.14 mg/mL) showed significant increase in mounting frequency, hormone levels, sperm count and histopathology which revealed the ruptured seminiferous tubules. These investigations confirmed significant intensification of sexual activities in experimental animals.