Anxiolytic effects of Leishmania braziliensis proteins in a zebrafish animal model

Abstract

Introduction. Leishmaniasis is among the 22 most neglected diseases worldwide. Few information is related about the evidence of neurological involvement that leishmaniasis can cause due to the ulcerations formed in the skin along short and long term. However, some authors have cited that leishmaniasis can cause inflammation in the central nervous system, which can trigger neuropsychomotor processes such as anxiety. Objective. The present study focuses on the evaluation of the effects of total Leishmania proteins on the central nervous system in the Zebrafish model. Method. Leishmania was initially cultured to extract total proteins by Sonication and Bradford methods. After total protein extraction, the zebrafish were inoculated to observe the symptomatology caused by protein load, and after seven days of inoculation, we performed the behavioral tests (Open Field and Bright & Dark). Results. The fish treated with Leishmania protein showed several cutaneous alterations, as well as behavioral changes. The open field behavioral tests showed a high number of crosses compared to the control group. In the light and dark test, the zebra fish had a longer stay in the light field, and an anxiolytic action was observed in the animals that received the protein administration. Conclusion. The total Leishmania proteins may have an anxiolytic action in the zebrafish model.