Sastric Siddha Drug against Paracetamol Induced Hepatotoxicity in Zebra Fish (Danio rerio) Model: A Scientific Validation Study

Abstract

The present study attempt to evaluate the hepatoprotective effect of MNK in the Zebrafish model using paracetamol induced hepatotoxicity. Numerous studies have confirmed that zebrafish and mammalian toxicity profiles are strikingly similar and the transparency of larval zebrafish permits direct in vivo assessment of drug toxicity including hepatotoxicity in zebrafish. Pharmacological studies were carried out in the adult zebra fish model in the category of four groups; each individual group contained ten animals. Groups 1, 2, 3, and 4 were considered as control groups, disease control groups, and drug treated groups at different concentrations, respectively. Histopathological analysis was recorded. The observed research findings were confirmed that this novel Siddha formulation MNK possess potent hepatoprotective effect at the both dose level of 250 mg/l–500 mg/l against paracetamol induced toxicity in Zebrafish. Pharmacological research findings confirms that the aqueous extract of Phyllanthus niruri exhibits hepatoprotective effects against carbon tetrachloride induced liver toxicity in mice. The herbals T. terrestris and Bauhinia acuminata present in this formulation were already documented for its immunomodulatory effects. Among all diseases, liver disorders are the most common. Although there are many powerful medications for liver diseases, there is still a need for affordable medications without side effects. According to the results of the study, the drug MNK has promising dose-dependent hepatoprotective activity and restores the fundamental liver architecture through its rejuvenating potential against paracetamol-induced toxicity in Zebrafish models. Further study is required to identify the hepatoprotective constituents present in the formulation as well as elucidating the mechanism of action.