Anti-wrinkle and anti-inflammatory effects of a combination of topically applied horse oil and dietary enzyme hydrolysates from horse bone

Abstract

This study investigated the anti-wrinkle and anti-inflammatory effects of horse bone hydrolysates of <3 kDa (HL) and topically applied horse oil (HO) on UVB-induced photoaging and inflammation in hairless mice. Forty-nine mice were divided into seven groups: −NC, chow without UVB irradiation; +UC, chow with UVB irradiation, +HLL, chow with low dose (500 mg/kg body weight (BW)) HL and UVB irradiation; +HLH, chow with high dose (1000 mg/kg BW) HL and UVB irradiation; +HO, chow and combined with topical application of HO and UVB irradiation; +HOHLL, treated with a combination of dietary HLL and topical application of HO with UVB irradiation; +HOHLH, treated with a combination of dietary HLH and topical application of HO with UVB irradiation. The transepidermal water loss in mice of all treatment groups was lower than that of mice in the +UC group. Furthermore, the wrinkle depth in the +HLH group was significantly lower than that in the +UC group. In addition, HO and low level of HL significantly decreased the level of tumor necrosis factor-α, interleukin-6, and matrix metalloproteinase-9, thereby down-regulating inflammation, including the cyclooxygenase-2 synthesis. In the future, human trials are needed to confirm the efficacy and synergistic effect of HL and HO.