Abstract
BackgroundBaicalin, the main metabolic component of Scutellaria baicalensis Georgi, has various pharmacological properties including anti-inflammatory, anti-oxidant, anti-apoptotic, anti-bactericidal and anti-viral. The purpose of this study was to investigate the anti-Marek’s disease virus (MDV) activities of baicalin in CEF cells.ResultsHere, we showed that baicalin could inhibit viral mRNA, protein levels and overall plaque formation in a time-dependent manner. We also found that baicalin could consistently inhibit MDV replication and directly affect the virus infectivity. Moreover, baicalin treatment has no effect on expression level of antiviral cytokine and inflammatory cytokines in MDV infected CEFs.ConclusionsThese results demonstrate that baicalin could be a potential drug against MDV infection.
Highlights
Baicalin, the main metabolic component of Scutellaria baicalensis Georgi, has various pharmacological properties including anti-inflammatory, anti-oxidant, anti-apoptotic, anti-bactericidal and anti-viral
Antiviral activity of baicalin on replication of Marek’s disease virus (MDV) in Chicken embryo fibroblast (CEF) cells After 96 h of inoculation, it was found that the transcription levels of the Meq and gB genes were significantly decreased at concentration of 20 μg/mL compared with the control group (Fig. 2a)
The 2 μg/mL low-dosage group did not show any significant inhibitory effect. These results indicate that 20 μg/mL baicalin has significant antiviral activity against MDV RB-1B infection in CEF cells
Summary
The main metabolic component of Scutellaria baicalensis Georgi, has various pharmacological properties including anti-inflammatory, anti-oxidant, anti-apoptotic, anti-bactericidal and anti-viral. We found that baicalin could consistently inhibit MDV replication and directly affect the virus infectivity. Baicalin treatment has no effect on expression level of antiviral cytokine and inflammatory cytokines in MDV infected CEFs. Marek’s disease virus (MDV) is a member of alpha-herpes virus subfamily, and serotype 1 strains are the etiologic agentof Marek’s disease (MD), which is a highly contagious and infectious malignant lymphoid neoplastic disease. MD is a neoplastic disease of chickens and other gallinaceous birds. It is one of the major diseases that threatens poultry industry worldwide and causes heavy economic losses [1]. In MD vaccine can prevent tumorigenesis and immunosuppression, but the virus can still replicate into fully infectious virus particles and shed cell free mature
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