Effect of IL-10 Expression on Pathogenesis of Nasal Polypogenesis in the Patients with Chronic Rhinosinusitis with Nasal Polyp

Abstract

RationaleEffect of IL-10 expression on pathogenesis of nasal polypogenesis in the patients with chronic rhinosinusitis with nasal polyp.MethodsA total of 42 subjects were enrolled in this study (10 control subjects, 32 chronic rhinosinusitis subjects. Nasal polyp tissue and uncinated tissue were collected during surgical procedure. Real-time PCR, immunohistochemistry and phagocytosis assay were performed on the sampled tissue.ResultsThe expression levels of the inflammatory cytokines including IL-10, IL-25, IL-17A and IFN-γ were significantly higher in the tissues from subjects with chronic rhinosinusitis with nasal polyp compared to the subjects with chronic rhinosinusitis without nasal polyp and control subjects. And there were strongly positive correlation between IL-10 and other inflammatory cytokines. However, the ratios of the expression level of IL-10 to the expression levels of other inflammatory cytokines except IL-25 were significantly lower in the tissues from subjects with chronic rhinosinusitis with nasal polyp compared to the subjects with chronic rhinosinusitis without nasal polyp and control subjects. We also found that the phagocytosis ability was weaker in the tissues from subjects with chronic rhinosinusitis with nasal polyp compared to the subjects with chronic rhinosinusitis without nasal polyp and control subjects.ConclusionsOur findings suggested that the imbalance of chronic, local immune responses may play important roles in the pathogenesis of chronic rhinosinusitis with nasal polyp. RationaleEffect of IL-10 expression on pathogenesis of nasal polypogenesis in the patients with chronic rhinosinusitis with nasal polyp. Effect of IL-10 expression on pathogenesis of nasal polypogenesis in the patients with chronic rhinosinusitis with nasal polyp. MethodsA total of 42 subjects were enrolled in this study (10 control subjects, 32 chronic rhinosinusitis subjects. Nasal polyp tissue and uncinated tissue were collected during surgical procedure. Real-time PCR, immunohistochemistry and phagocytosis assay were performed on the sampled tissue. A total of 42 subjects were enrolled in this study (10 control subjects, 32 chronic rhinosinusitis subjects. Nasal polyp tissue and uncinated tissue were collected during surgical procedure. Real-time PCR, immunohistochemistry and phagocytosis assay were performed on the sampled tissue. ResultsThe expression levels of the inflammatory cytokines including IL-10, IL-25, IL-17A and IFN-γ were significantly higher in the tissues from subjects with chronic rhinosinusitis with nasal polyp compared to the subjects with chronic rhinosinusitis without nasal polyp and control subjects. And there were strongly positive correlation between IL-10 and other inflammatory cytokines. However, the ratios of the expression level of IL-10 to the expression levels of other inflammatory cytokines except IL-25 were significantly lower in the tissues from subjects with chronic rhinosinusitis with nasal polyp compared to the subjects with chronic rhinosinusitis without nasal polyp and control subjects. We also found that the phagocytosis ability was weaker in the tissues from subjects with chronic rhinosinusitis with nasal polyp compared to the subjects with chronic rhinosinusitis without nasal polyp and control subjects. The expression levels of the inflammatory cytokines including IL-10, IL-25, IL-17A and IFN-γ were significantly higher in the tissues from subjects with chronic rhinosinusitis with nasal polyp compared to the subjects with chronic rhinosinusitis without nasal polyp and control subjects. And there were strongly positive correlation between IL-10 and other inflammatory cytokines. However, the ratios of the expression level of IL-10 to the expression levels of other inflammatory cytokines except IL-25 were significantly lower in the tissues from subjects with chronic rhinosinusitis with nasal polyp compared to the subjects with chronic rhinosinusitis without nasal polyp and control subjects. We also found that the phagocytosis ability was weaker in the tissues from subjects with chronic rhinosinusitis with nasal polyp compared to the subjects with chronic rhinosinusitis without nasal polyp and control subjects. ConclusionsOur findings suggested that the imbalance of chronic, local immune responses may play important roles in the pathogenesis of chronic rhinosinusitis with nasal polyp. Our findings suggested that the imbalance of chronic, local immune responses may play important roles in the pathogenesis of chronic rhinosinusitis with nasal polyp.