Antispasmodic activity of &lt;i&gt;Symplocos paniculata&lt;/i&gt; is mediated through opening of ATP-dependent K+ channel

Khalid Hussain Janbaz,Mamoona Khalid,Fatima Saqib,Saba Akram

doi:10.3329/bjp.v11i2.24667

Antispasmodic activity of Symplocos paniculata is mediated through opening of ATP-dependent K+ channel

Khalid Hussain Janbaz, Mamoona Khalid + Show 2 more

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https://doi.org/10.3329/bjp.v11i2.24667

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Abstract

Symplocos paniculata is a medicinal plant used by native healers to manage gastrointestinal ailments. The crude methanolic extract of S. paniculata was screened pharmacologically both in vitro and in vivo for the validation of its therapeutic potential. It suppressed the spontaneous activity of isolated rabbit jejunum preparations and also caused inhibition of the low K+ (20 mM)- induced spastic contractions in isolated rabbit jejunum preparations in a manner comparable to cromakalim. The relaxant effect was found to be blocked following glibenclamide exposure of the isolated tissue preparations similar to cromakalim, suggesting that observed response was likely to be mediated through opening of ATP dependent K+ channels. Following oral administration to mice provided protection against castor oil-induced diarrhea in a manner similar to loperamide. The plant material was found safe in toxicity study up to oral dose of 8 g/kg in mice. Hence, present study provides a scientific basis for the vernacular use of S. paniculata in gastro-intestinal system.

Highlights

Symplocos paniculata (Syn: S. chinensis, Pronus paniculata and S. crataegoides; Family: Symplocaceae), locally known as Ludh pathani, is a small tree distributed throughout the world but grows luxuriously in moist and moderate climatic conditions prevailed in Northern areas of Pakistan (Dymock et al, 1972)
The low K+ (20 mM)-induced spastic contraction in isolated rabbit jejunum preparation were completely relaxed on bath in concentration-dependent manner (0.03-5 mg/mL) (Figure 2)
The observed relaxant effect was speculated to be mediated through K+-channel opening activity as cromakalim caused suppression of the spontaneous contractions and caused relaxation of low K+ (20 mM)-induced spastic contractions in isolated rabbit jejunum preparation

Summary

Introduction

Symplocos paniculata (Syn: S. chinensis, Pronus paniculata and S. crataegoides; Family: Symplocaceae), locally known as Ludh pathani, is a small tree distributed throughout the world but grows luxuriously in moist and moderate climatic conditions prevailed in Northern areas of Pakistan (Dymock et al, 1972). Phytochemical investigations revealed presence of monomethyl pelargonidine glucosides, loturine, collutrine, loturidine, oxalic acid, pytosterols, 3-monoglucofuranoside, betulin, acetyloleanolic acid, oleanolic acid, ellagic acid (Joy et al, 2001), octacos-1-ene, stigmasterol, lupeol and salirepin (Kumar and Jangwan, 2012)

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