Year-end Sale % OFF 
Abstract
Snake envenomation is a major cause of death and disability in many developing countries. Neocarya macrophylla (Sabine) Prance ex F. White (Malpighiales: Chrysobalanaceae) have been reportedly used in traditional medicine to treat snake envenomation. Bioassay-guided isolation of antivenom principles was carried out on the leaf of N. macrophylla against Naja nigricollis venom. The methanol extract of N. macrophylla leaf and its ethylacetate and n-butanol fraction significantly (P < 0.05) protected mice against venom-induced lethality with 100% survival rate and there was remarkable inhibition of the poisonous effects of PLA2 enzyme by the extracts and the fractions. Encouraged by this result, the ethylacetate soluble fraction was subjected to purification using vacuum liquid chromatography and gel filtration which led to the isolation of quercetin as the bioactive principle. The identity of the compound was determined on the basis of chemical tests, and by comparison of its 1H-NMR data with literature, this is the first report of isolation of this compound from the leaf of the plant. However, the results of the study suggests that the leaf of N. macrophylla possess significant antisnake venom activity which provide the scientific basis for its use in traditional treatment of snakebites.
Highlights
Snakebites is a neglected public health concern in many developing countries (Alirol et al, 2010; Chippaux, 2011) especially in the rural areas where access to healthcare facilities is severely limited (Aleku et al, 2014; Dechambenoit, 2016)
We have recently demonstrated the antimicrobial activity of stigmasterol isolated from N. macrophylla, exhibiting broad spectrum antimicrobial activity thereby validating its use for the treatment of infectious diseases (Yusuf et al, 2018)
The antisnake venom activity of the methanol leaf extract of N. macrophylla and against Naja nigricollis venom and isolation of quercetin, a known flavonoid
Summary
Snakebites is a neglected public health concern in many developing countries (Alirol et al, 2010; Chippaux, 2011) especially in the rural areas where access to healthcare facilities is severely limited (Aleku et al, 2014; Dechambenoit, 2016). Many snakebite patients rely on alternative herbal medicinal antivenom products with no or limited scientific evidence of safety and efficacy. Nigeria to treat several diseases, including infectious diseases and snake bites (Yusuf et al, 2015a). The ease of availability of this plant in Nigeria and elsewhere prompted us to conduct antisnake venom activity and phytochemical analysis of the stem bark of N. macrophylla in which stigmasterol and bis-(5,7-diacetyl-catechin-4’-αrhamnopyranoside) were isolated (Yusuf et al, 2015a; Yusuf et al, 2015b). We have recently demonstrated the antimicrobial activity of stigmasterol isolated from N. macrophylla, exhibiting broad spectrum antimicrobial activity thereby validating its use for the treatment of infectious diseases (Yusuf et al, 2018)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
